Txoj Kev Tshawb Nrhiav Kev Tiv Thaiv Kab Mob Ntawm Tsiaj
May 20, 2019
Hauv vitro xeem txoj hauv kev rau cov plaub hau lig-cov plaub hau ua xua Kev kuaj tawv nqaij thiab kev tiv thaiv kev tiv thaiv kab mob yog ob txoj hauv kev los kuaj cov plaub hau lig-inducing allergic reactions (DTH).
Hauv kev ntsuam xyuas ntawm daim tawv nqaij, ib qho kev rov- teb rau cov tshuaj tiv thaiv uas tau ua rau tus neeg mob rhiab heev, thiab kev sib cuag tsis haum yog qhov muaj peev xwm los ntsuas tus neeg tau txais kev muaj peev xwm ua rau muaj kev nkag siab rau cov khoom uas tsis tau raug pom dua . 1. Kev kuaj tawv nqaij nrog kev kuaj tawv nqaij DTH, feem ntau siv cov tshuaj tiv thaiv kab mob TB muaj cov kab mob TB ntshiab protein derivatives (PPD, virus), thiab lwm yam. nyob rau hauv tib neeg kev ntsuam xyuas nyob rau hauv lub forearm ntawm daim tawv nqaij txhaj tshuaj ntawm ib tug me me ntawm soluble antigen, 24 mus rau 48 me me, ntsuas qhov loj ntawm liab thiab o tawv pob txha, tawv pob caus txoj kab uas hla ntau tshaj 10 hli yog pom zoo. Nws qhia tau hais tias qhov kev kawm muaj qee qhov kev tiv thaiv kab mob ntawm cov kab mob, yog tias qhov kev ntsuam xyuas ntawm daim tawv nqaij tsis teb, tuaj yeem siv ntau dua ntawm cov tshuaj tiv thaiv antigen, yog tias tseem tsis muaj cov lus teb tsis zoo, nws yuav tsum tsis suav nrog kev kuaj tawv nqaij kev ua yuam kev, kuj tseem yuav yog qhov kev kawm tsis tau raug rau cov tshuaj tiv thaiv kab mob no, kuj tuaj yeem yog vim lub cev tsis muaj zog tiv thaiv kab mob, los yog kev ua haujlwm ntawm cellular.
los yog tsis yog -reactive vim muaj kab mob loj ( qhua pias, mob TB diffuse). 2. Kev tiv thaiv kab mob feem ntau siv cov tshuaj uas tsis muaj molecular hnyav xws li dinitrol chlorobenzene (DNCB) induced contact allergy. Cov tshuaj khi rau ntawm daim tawv nqaij cov protein kom ntxias DTH cov tshuaj tiv thaiv. Hauv kev kuaj tsiaj, daim tawv nqaij zoo nkaus li yog tias daim tawv nqaij zoo tom qab 7 mus rau 10 hnub ntawm kev txhawb nqa tom qab thawj zaug DNCB raug siv rau ntawm daim tawv nqaij.
Qhov kev sim no tsis siv los ntawm tib neeg lawm.
Hauv vitro tshawb nrhiav txoj kev tiv thaiv kab mob hauv vitro tshawb nrhiav cov lej thiab kev ua haujlwm ntawm lymphocytes, yooj yim dua los sau cov ntshav kuaj, ua ntej ntawm tag nrho cov yuav tsum tau cais lossis ntxuav cov lymphocytes, feem ntau siv glutalyson -panphotonic glutamine nrog ib feem ntawm 1.077 lymphocytes stratification kua, nyob rau sab saum toj ntawm cov ntshav lymphocytes. stratified kua centrifugation, vim cov qe ntshav liab (1.092), polymorphic nuclear dawb cov qe ntshav (feem ntau 1.090), cov proportions ntawm lymphocytes (1.070) yog sib txawv thiab sib cais los ntawm ib leeg. Lymphocytes thiab monocytes tsim ib txheej nyias ntawm qhov sib txuas ntawm plasma thiab stratification.
Ua tib zoo faib cov txheej nyias nyias ntawm cov hlwb, ntawm cov lymphocytes suav txog 80%, mononucleosis suav txog 20%, lymphocytes suav txog 80%, B hlwb suav txog 4% txog 10%, uas tsis yog-DT, tsis yog-B hlwb. ⑴ E-garland: tib neeg T cell nto muaj SRBC receptor (CD2) tuaj yeem khi rau SRBC los ua ib lub nplhaib rose-zoo li cov qauv, RBM ncua kev kawm ntawv thiab SRBC cais los ntawm cov kua nplaum sib xyaw hauv cov kua qaub uas muaj cov ntshav qab zib, kab lis kev cai ntawm 37 degrees C 5 mus rau 4 degrees Csp hmo ntuj, suav nrog Cov ntshav peripheral lymphocytes nyob rau hauv li ntawm 70% mus rau 80% ntawm lymphocytes tsim wreaths, los yog T hlwb.
Tam sim no txoj kev no tau siv los cais T hlwb yam tsis tas yuav ua T- suav xov tooj. (2) suav T hlwb nrog cov tshuaj tiv thaiv monoclonal: tib neeg PBM tau muab faib ua peb qhov sib npaug, ua ntej -cov tshuaj tiv thaiv kab mob ntawm nas anti- tib neeg CD3, CD4 thiab CD8 tau ua ke nrog cov hlwb, thiab tom qab ntawd cov tshuaj tiv thaiv thib ob ntawm luav anti-nas IgG sau los ntawm FITC yog siv rau indirect immunofluorescent staining, Hauv cov txiaj ntsig ntawm fluorescence microscope lossis flow cytometer test, hauv PBM cd3 antibodies dyed fluorescent cells hu ua CD3-plus cells, lossis tag nrho T hlwb. Cov neeg ib txwm nyob hauv PBM T hlwb suav txog 70% txog 80%. Cov txiaj ntsig ntawm CD4-plus hlwb thiab CD8-hlwb hauv tib neeg yuav tsum ua raws li cov CD3-cells. Qhov piv ntawm CD4-plus hlwb rau CD8-hlwb yog kwv yees li 2/1 rau cov neeg ib txwm muaj, thaum qhov piv ntawm cov neeg mob AIDS yog tsawg dua 1.7.