Canine Distemper Test Paper Test Principple Thiab Test Procedure Cov ntsiab lus piav qhia

Canine distemper test paper tuaj yeem tshawb xyuas sai, raug thiab yooj yim kuaj CDV antigens hauv dev qhov muag zais cia lossis conjunctiva, qhov ntswg qhov ntswg, qaub ncaug, qhov chaw mos thiab qhov quav ( quav) thiab ntshav. Nws yuav tsum muaj qhov ntsuas me me ntawm lub cev, lub sijhawm luv (tsuas yog 20 feeb), thiab nkag siab yooj yim.

Qhov muag secretions los yog antigens nyob rau hauv conjunctiva, qhov ntswg, qaub ncaug, qhov chaw mos thiab qhov quav (feces)

CDV antigen hauv cov ntshav

Kev sau thiab khaws cov khoom uas yuav tsum tau khaws: Cov ntshav, ntshav, cov kua dej hauv lub qhov muag thiab cov qog nqaij hlav hauv qhov ntswg yog siv los ua cov khoom uas yuav tsum tau sau, thiab yuav tsum muaj 100ul ntawm cov khoom. Cov khoom ntsuas tuaj yeem khaws cia rau 7 hnub ntawm 2-8 degrees. Yog tias cov tshuaj ntsuam xyuas tau khaws cia ntev, cov ntshav los yog ntshav yuav tsum tau muab cais thiab khaws cia ntawm -20 degrees. Thaum ua qhov kev xeem, nws yuav tsum tau muab tso rau hauv ib qho chaw sov so kom qhov kub thiab txias ntawm cov khoom kuaj tau zoo raws li chav tsev kub ua ntej ua qhov kev xeem.

Txoj kev kuaj pom: Tshem cov khoom siv tawm ntawm lub hnab thiab muab tso rau hauv qhov chaw kab rov tav ntawm chav sov li 10 feeb. Cov tshuaj tiv thaiv phaj tau muab tso rau hauv qhov chaw ntub dej, uas tuaj yeem ua rau poob siab thiab ruaj khov ntawm cov tshuaj tiv thaiv kom txo qis, yog li nws yuav tsum tau qhib thiab muab rho tawm ua ntej siv. Qhib cov ntawv nyiaj, muab cov cuab yeej tso rau hauv av, thiab sau cov ntaub ntawv ntsig txog tus tsiaj cuam tshuam ntawm lub cuab yeej. Yog hais tias cov khoom kuaj yog cov ntshav los yog ntshav, siv ib lub dropper los tso 2-3 tee (100ul) ntawm cov khoom kuaj. Yog tias nws yog qhov muag tawm (lub kua muag) lossis cov hnoos qeev hauv qhov ntswg, siv cov paj rwb swab rau cov qauv los sau cov khoom kuaj thiab dilute nws nyob rau hauv lub dilution buffer, thiab tom qab ntawd tso 2-3 tee rau ntawm qhov chaw kuaj khoom. Tom qab 5-10 feeb, txiav txim qhov kev xeem. Tom qab tso cov khoom sim, tos cov khoom sim kom tag nrho ntawm cov tshuaj tiv thaiv phaj, thiab txiav txim hauv 10 feeb, thiab cov tshuaj tiv thaiv tom qab 10 feeb yog suav tias yog qhov tsis zoo.

Qhov tshwm sim tsis zoo: Hauv qhov tshwm sim, thaum tsuas yog "C kab" tshwm sim, nws txhais tau hais tias CDV antigen tsis zoo, qhia tias tus dev tsis muaj canine distemper.

Cov txiaj ntsig zoo: Hauv qhov tshwm sim, thaum "C kab" thiab "T kab" tshwm sim tib lub sijhawm, nws txhais tau hais tias CDV antigen yog qhov zoo, qhia tias tus dev tau kis tus kab mob canine distemper.

Cov txiaj ntsig tsis raug: Thaum "C kab" tsis tshwm sim lossis tsis pom "C kab" lossis "T kab" tshwm sim, qhov kev xeem tsis raug.

Yog vim li cas rau qhov tshwm sim no yuav siv tsis raug lossis cov khoom nws tus kheej tau ua tsis tiav thiab yuav tsum tau sim dua.

Txiav txim qhov tsis zoo thiab qhov zoo raws li cov txiaj ntsig ntawm cov kab xeem thiab cov kab tswj.

"T kab" --- qhov kev txiav txim ntawm kab (paub meej seb tus qauv puas muaj CDV antigen).

"C kab" --- kev kuaj xyuas kab (kom paub meej tias cov qauv no ib txwm nthuav dav hauv cov khoom siv).

CDV---English abbreviation for Canine Distemper Virus.

Tsis zoo: Tsuas yog thaum kab tswj (C) liab lossis ntshav. Tus mob no txhais tau hais tias tsis muaj tus kab mob canine distemper.

Qhov zoo: Tsuas yog thaum kab xeem (T) thiab cov kab tswj (C) ob leeg liab lossis ntshav. Qhov xwm txheej no txhais tau tias koj tau kis tus kab mob canine distemper.

Kev ceev faj rau kev siv

1. Siv nruj raws li txoj kev teev tseg hauv cov khoom siv.

2. Daim ntawv xeem no tsuas yog siv rau kev kuaj CDV antigen.

3. Thaum qhov kev txiav txim siab tsis zoo, nws tsis paub tseeb tias tsis muaj CDV antigen. Yog tias tsim nyog, lwm txoj hauv kev tuaj yeem siv los lees paub.

4. Txhawm rau txhim kho qhov tseeb, nws raug nquahu kom siv ntau cov qauv thiab cov qauv sib xyaw.

5. Tag nrho cov qauv yuav tsum raug kho raws li qhov yuav kis tau.

6. Cov txiaj ntsig ntawm cov khoom siv tsuas yog siv rau cov kws kho mob siv. Cov txiaj ntsig kev kuaj mob yog txiav txim los ntawm tus kws kho mob ua ke nrog cov tsos mob ntawm kev kho mob.

7. Tom qab cov khoom siv tau tawm ntawm lub tub yees, nws yuav tsum tau siv tom qab qhov kub thiab txias rov qab los rau hauv chav tsev. Thiab siv nws sai li sai tau tom qab qhib.

8. Cov qauv tom qab kuaj yuav tsum tau siv sai li sai tau.

9. Tsis txhob siv cov khoom siv uas siv.

10. Thaum khaws cia, thov ceev faj tsis txhob muab tso rau qhov chaw me nyuam mus txog.

11. Cov khoom siv tsim nyog rau kev cia ntawm 2-8 degrees (cov tub yees cia). Tsis txhob khov, thiab tsis txhob muab cov khoom tso rau hauv qhov chaw tshav ntuj ncaj qha lossis qhov chaw kub.