Cov Ntsiab Cai ntawm ELISA Cov Khoom Siv

Lub hauv paus ntawm immunoassay thev naus laus zis yog nyob rau hauv cov tshuaj tiv thaiv tshwj xeeb ntawm cov tshuaj tiv thaiv kab mob antigen, yog li txhua qhov siab -cov tshuaj tiv thaiv kab mob zoo tsis tuaj yeem raug cais los ntawm cov khoom zoo -cov ntaub ntawv zoo, xws li antigen antibodies, enzymes, thiab lwm yam. Antigens yav dhau los siv rau immunoassay feem ntau yog purified antigens, thaum cov tshuaj tiv thaiv kab mob monoclonal. cov tshuaj tiv thaiv tau txais tom qab purified antigen immunoanimals thiab monoclonal antibodies tau siv hybrid qog cov tswv yim, thaum antigens los yog antibody markers xws li enzyme-labeled bindings yog npaj los ntawm ntau yam tshuaj synthesis txoj kev.

Nyob rau hauv xyoo tas los no, nrog rau kev loj hlob ntawm molecular biology, kev siv genetic engineering txoj kev los npaj ntau yam tshwj xeeb antigens los yog cov tshuaj tiv thaiv kab mob thiab lawv cov enzyme bindings thiab lwm yam immunoassay reagents dhau los ua ib tiam tshiab ntawm reagents, ntau yam tshiab ntawm kev siv tshuaj ntsuam xyuas txoj kev kuj tshwm sim. Cov yam ntxwv ntawm HBsAg reagent (kev kuaj xyuas hom: kev kho mob antibody sandwich txoj kev): lub pob yog tshuaj tiv thaiv rau tshis ntau -tsis kam. Poly-kuj muaj kev sib raug zoo siab thiab rov ua haujlwm rau lub rooj qub. Enzyme deg cov tshuaj tiv thaiv yog cov nas sib xyaw nrog cov chaw sib txawv nrog HBsAg, HBsAg cov qauv sib txawv tuaj yeem ntsuas tau, txhim kho kev kuaj pom tseeb (99.98%) txhawm rau txhim kho kev tshawb pom rhiab heev, daim ntawv thov Parl Ehrich txoj kev xav (PEI) HBsAg tus qauv, rhiab heev rau ad5ng tus qauv 0.0.

ml rhiab heev rau ay txheem cov khoom yog 0.025 ng / ml ELISA xeem cov khoom siv daim ntawv thov zoo sandwich immunotest tshuab, nrog hluavtaws HEV peptide antigen pob los ntawm micro- ntxeem tau phaj slats, cov peptides yog cov tseem ceeb amino acid sib lawv liag ntawm Suav heV strain ntawm heev heev antigens qhib lub thawv, peptide nyeem ntawv 2 thiab qhib kev nyeem ntawv lub thawv 3. Cov qauv lossis cov qauv khoom raug muab ntxiv rau hauv lub qhov thiab incubated, yog tias muaj cov tshuaj tiv thaiv HEV IgM, cov tshuaj tiv thaiv kab mob no yuav khi rau HEV polypeptide antigens thiab tsau rau saum lawv kom tshem tawm lwm cov tshuaj tiv thaiv tsis zoo thiab lwm yam hauv cov qauv. Tom qab ntawd ntxiv cov yaj los tiv thaiv -tib neeg IgM-HRP (ntsim cag peroxidase) enzyme khi, tom qab qhov thib ob incubation, cov enzyme khi yuav ua ke nrog thawj incubation binding HEV IgM antibody, ntxuav phaj kom tshem tawm cov uncombined enzyme binding, ntxiv TMB substrate tov yuav tshwm sim nyob rau hauv peb. enzyme-Cov tshuaj tiv thaiv hauv qab, Tsuas yog cov qhov nyob hauv cov khoom sib xyaw uas muaj HEV IgM cov tshuaj tiv thaiv thiab cov enzyme khi yuav hloov xim, ntxiv cov tshuaj sulfuric acid kom tshem tawm cov tshuaj tiv thaiv ntawm cov enzyme thiab cov substrate, thiab ntsuas OD tus nqi ntawm 450nm, raws li tus qauv xeem ntawm no HEV IgM antibody elisa cov khoom siv ntau dua, ntau dua li O. txiav-Cov nqi tawm yog suav tias yog thawj qhov kev kuaj pom zoo.