Cov lus ceeb toom hauv cov txheej txheem kuaj cov khoom siv ELISA

1, txhawm rau xyuas qhov tseeb ntawm rab phom pipetting, qhov yuam kev tsis tuaj yeem tshaj 2%. Nws tuaj yeem txiav txim siab siv dej thiab hluav taws xob sib npaug. Tab sis nws yog qhov zoo tshaj kom muaj cov kws tshaj lij los kho nws.

2, Yuav tsum tau nruab nrog 20ul, 50ul, 100ul, 1000ul thiab ib qho. Tom qab kos cov kua sib txawv, hloov lub taub hau ntawm rab phom. Txawm tias thaum kos duab rau cov qauv.

3, mus rau qhov ntsuas 1 teev ua ntej cov khoom siv tawm ntawm lub tub yees, kom txhua yam reagents rov qab los rau hauv chav tsev kub, kom cov txiaj ntsig tau ruaj khov.

4, Kev sim, ua kom lub substrate kom tsis txhob muaj teeb pom kev zoo.

5, Nrog rau rab phom kom nqus tau cov kua ceev tsis tuaj yeem ceev heev, thiaj li tsis tsim cov npuas thiab ua kom qhov nqus dej tsis raug.

6, Thaum nqus cov kua dej, siv qhov ntau thiab tsawg ntawm qhov ze ntawm rab phom kom nqus, txo qhov yuam kev.

7, Thaum ntxiv cov kua rau lub qhov enzyme marker qhov, tsis txhob sib cuag ntawm lub taub hau ntawm rab phom thiab cov kua hauv lub qhov, tuaj yeem ua rau cov tee dej ntawm lub taub hau ntawm rab phom thiab lub qhov phab ntsa sib cuag, cov tee dej yuav ntws tawm.

8, Cov kua yog tag nrho ntxiv, lub phaj enzyme tuaj yeem maj mam wiggled ntawm lub rooj nyob rau hauv parallel rau 30 vib nas this, sib tov kua. Nws tseem tuaj yeem siv qhov kev ua haujlwm swaying ntawm enzyme marker.

9, da dej sov, siv cov ntawv nplaum lossis daim kab xev kaw enzyme phaj, kom tiv thaiv cov dej evaporation.

10, Ntxuav lub phaj, txhua qhov ntxuav ntxiv, yuav tsum tau tso tseg rau 1 feeb, kom ntxuav tau ntau dua. Thaum tsis muaj phaj ntxhua khaub ncaws, ncuav cov kua, mus rau lub phaj enzyme nyob rau hauv cov ntawv xov xwm los yog ntaub plaub daim ntawv nyuaj patting qhuav.

11. Thaum cov tshuaj pleev ib ce tsis txaus, phosphate buffer ntawm PH7.4,0.02M tuaj yeem tsim los ntawm cov dej distilled, thiab 0.1% twain 20 yog ntxiv rau cov tshuaj pleev ib ce. Ntxiv 1/1000 ntawm sodium nitrogen pawg kom ntev -lub sij hawm khaws cia.

12, Lub substrate yog lub teeb rhiab heev, npaj rau kev siv ua ntej tam sim no.

13, Ua ntej kuaj pom, qhib lub enzyme marker, kom nws ruaj khov rau ntau tshaj 10 feeb.

14, Lub substrate muaj ib tug tej yam toxicity, txiav kua rau ntawm daim tawv nqaij yog corrosive, yuav tsum tau sim kom tsis txhob sib cuag.

15, Cov qauv yuav tsum tau kuaj xyuas kom meej, txwv tsis pub nws yuav cuam tshuam cov txiaj ntsig.

16, Lub sij hawm da dej sov yuav tsum ua raws li cov cai ntawm cov khoom siv.

17, Yuav tsum sim ua ob - qhov kev sim, thiaj li ua kom cov ntaub ntawv raug.

18, Cov txiaj ntsig ntawm cov qauv hauv kev tsis ntseeg yuav raug lees paub los ntawm lwm txoj hauv kev.